Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen

Severino de Paiva Sobrinho; Maria Cristina de Figueiredo e Albuquerque; Petterson Baptista da Luz; Leonarda Grillo Neves; Elisangela Clarete Camili

doi:10.4336/2017.pfb.37.92.1145

Authors

Severino de Paiva Sobrinho Universidade do Estado de Mato Grosso
Maria Cristina de Figueiredo e Albuquerque Universidade Federal do Mato Grosso
Petterson Baptista da Luz Universidade do Estado de Mato Grosso
Leonarda Grillo Neves Universidade do Estado de Mato Grosso
Elisangela Clarete Camili Universidade Federal do Mato Grosso

DOI:

https://doi.org/10.4336/2017.pfb.37.92.1145

Keywords:

Genipa americana, Germplasm conservation, Seed preservation

Abstract

The objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 Ã— 2 (cryoprotectant concentrations Ã— thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide.

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Author Biography

Severino de Paiva Sobrinho, Universidade do Estado de Mato Grosso

Graduado em Agronomia e Doutor em Agricultura Tropical - área de cocnetração sementes. Professor Adjunto no Curso de Agronomia da UNEMAT

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