In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
DOI:
https://doi.org/10.4336/2019.pfb.39e201901895Keywords:
Micropropagation, Growth regulators, AnnonaceaeAbstract
The purpose of this study was to develop an in vitro multiplication methodology for Xylopia aromatica, aiming the production of seedlings, evaluating culture media and concentrations of more suitable growth regulators. Nodal segments from seedlings produced from seeds and kept in a nursery were used as explants. The Murashige & Skoog (MS) and Wood Plant Medium (WPM) culture media were tested supplemented with 800 mg L-1 of polyvinylpyrrolidone (PVP), 30 g L-1 of sucrose, 0.054 µM of naphthaleneacetic acid (NAA), 6 g L-1 of agar and concentrations of 2.22 and 3.55 µM of benzylaminopurine (BAP) for in vitro multiplication. At 30 days, the number of lateral sprouts emitted and percentage of explants forming callus at the base were evaluated. The highest multiplication rates were obtained on MS medium supplemented with 3.55 µM of BAP. Regarding callus formation, the MS medium promoted greater formation than WPM medium, however it did not compromise the formation of shoots. It was possible to establish the introduction and multiplication phases, thus defining the culture medium and concentrations of growth regulators most suitable for in vitro culture of Xylopia aromatica.Downloads
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References
Brondani, G. E. et al. Desinfestação e meio de cultura para o Estabelecimento in vitro de segmentos nodais de Liquidambar styraciflua. Floresta, v. 40, n. 3, p. 541-554, 2010. http://dx.doi.org/10.5380/rf.v40i3.18916.
Cheng, Z. J. et al. Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3. Plant Physiology, v. 161, n. 1, p. 240-251, 2013. http://dx.doi.org/10.1104/pp.112.203166.
Deccetti, S. F. C. et al. La micropropagation d´Annona glabra L. Ã partir de segments nodaux. Fruits, v. 60, n. 5, p. 319-325, 2005.
Encina, C. L. et al. Biotechnology applied to Annona species: a review. Revista Brasileira de Fruticultura, v. 36, n. spe1, p. 17-21, 2014. http://dx.doi.org/10.1590/S0100-29452014000500002.
Ezeibekwe, I. O. et al. Effects of combination of diferente levels of Auxin (NAA) and Cytokinin (BAP) on in vitro propagation of Dioscorea rotundata L. (White Yam). New York Science Journal, v. 2, n. 5, p. 1-8, 2009. http://dx.doi.org/jmolgene.2009.18.22.
Freitas, R. T. et al. In vitro culture of Annona emarginata: a rootstock for commercial annonaceae species. Plant Cell Culture & Micropropagation, v. 12, n. 1, p. 1-6, 2016.
George, E. F. & Sherrington, P. D. (Ed.). Plant propagation by tissue culture. Eversley: Exegetics, 1984. 709 p.
Golle, D. P. et al. Estabelecimento e desenvolvimento in vitro de Eugenia involucrata DC.: influência do tipo de explante e do meio nutritivo. Ciência Florestal, v. 22, n. 1, p. 207-214, 2012. DOI: 10.5902/198050985092.
Grattapaglia, D. & Machado, M. A. Micropropagação. In: Torres, A. C. et al. Cultura de tecidos e transformação genética de plantas. Brasília, DF: EMBRAPA-SPI: EMBRAPA-CNPH, 1998. p. 183-260.
Kielse, P. et al. Regeneração in vitro de Parapiptadenia rigida. Ciência Rural, v. 39, n. 4, p. 1088-1094, 2009. DOI: 10.1590/S0103-84782009005000046.
Lloyd, G. & Mccown, B. Commercially feasible micropropagation of mountain laurel, (Kalmia latifolia), by use of shoot tip culture. Combined Proceedings: International Plant Propagator´s Society, n. 30, p. 421-427, 1981.
Lopes, S. F. et al. Caracterização ecológica e distribuição diamétrica da vegetação arbórea em um remanescente de floresta estacional semidecidual na Fazenda Experimental do Glória, Uberlândia, MG. Bioscience Journal, v. 27, p. 322-335, 2011.
Lorenzi, H. (Ed). Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Nova Odessa, SP: Editora Plantarum, 2014. 388 p.
Malosso, M. G. et al. Micropropagation and in vitro conservation of Jacaranda decurrens Cham. Journal of Medicinal Plants Research, v. 6, n. 7, p. 1147-1154, 2012. http://dx.doi.org/10.5897/JMPR11.436.
Miranda, N. A. et al. Meio de cultura, reguladores de crescimento e formas de vedação de tubos de ensaio na multiplicação in vitro de candeia (Eremanthus incanus (Less.) Less). Scientia Forestalis, v. 44, n. 112, p. 1009-1018, 2016. http://dx.doi.org/10.18671/scifor.v44n112.22.
Murashige, T. & Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, v. 15, p. 473-497, 1962. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x.
Nagori, R. & Purohi, S. D. In vitro plantet regeneration in Annona squamosa thought direct shoot but differentiation on hypocotyl segmen ts. Scientia Horticulturae, v. 99, p. 89-98, 2004. http://dx.doi.org/10.1016/S0304-4238 (03)00084-0.
Oliveira, L. S. et al. Micropropagação de espécies florestais brasileiras. Pesquisa Florestal Brasileira, v. 33, n. 76, p. 439-453, 2013. http://dx.doi.org/10.4336/2013.pfb.33.76.481.
Pinhal, H. F. et al. Aplicações da cultura de tecidos vegetais em fruteiras do Cerrado. Ciência Rural, v. 41, n. 7, p. 1136-1142, 2011. http://dx.doi.org/10.1590/S0103-84782011005000089.
Rodrigues, M. R. et al. Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.). In vitro Cellular & Developmental Biology: Plant, v. 48, n. 1, p. 67-72, 2012. http://dx.doi.org/10.1007/s11627-011-9398-8.
Socolowski, F. et al. Viability of recently harvested and stored Xylopia aromatica (Lam.) Mart. (Annonaceae) seeds. Revista Brasileira de Sementes, v. 34, n. 3, p. 408-415, 2012. http://dx.doi.org/10.1590/S0101-31222012000300007.
Xavier, A. et al. (Ed.). Silvicultura clonal: princípios e técnicas. Viçosa, MG: Ed. da UFV, 2013. 279 p.
Cheng, Z. J. et al. Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3. Plant Physiology, v. 161, n. 1, p. 240-251, 2013. http://dx.doi.org/10.1104/pp.112.203166.
Deccetti, S. F. C. et al. La micropropagation d´Annona glabra L. Ã partir de segments nodaux. Fruits, v. 60, n. 5, p. 319-325, 2005.
Encina, C. L. et al. Biotechnology applied to Annona species: a review. Revista Brasileira de Fruticultura, v. 36, n. spe1, p. 17-21, 2014. http://dx.doi.org/10.1590/S0100-29452014000500002.
Ezeibekwe, I. O. et al. Effects of combination of diferente levels of Auxin (NAA) and Cytokinin (BAP) on in vitro propagation of Dioscorea rotundata L. (White Yam). New York Science Journal, v. 2, n. 5, p. 1-8, 2009. http://dx.doi.org/jmolgene.2009.18.22.
Freitas, R. T. et al. In vitro culture of Annona emarginata: a rootstock for commercial annonaceae species. Plant Cell Culture & Micropropagation, v. 12, n. 1, p. 1-6, 2016.
George, E. F. & Sherrington, P. D. (Ed.). Plant propagation by tissue culture. Eversley: Exegetics, 1984. 709 p.
Golle, D. P. et al. Estabelecimento e desenvolvimento in vitro de Eugenia involucrata DC.: influência do tipo de explante e do meio nutritivo. Ciência Florestal, v. 22, n. 1, p. 207-214, 2012. DOI: 10.5902/198050985092.
Grattapaglia, D. & Machado, M. A. Micropropagação. In: Torres, A. C. et al. Cultura de tecidos e transformação genética de plantas. Brasília, DF: EMBRAPA-SPI: EMBRAPA-CNPH, 1998. p. 183-260.
Kielse, P. et al. Regeneração in vitro de Parapiptadenia rigida. Ciência Rural, v. 39, n. 4, p. 1088-1094, 2009. DOI: 10.1590/S0103-84782009005000046.
Lloyd, G. & Mccown, B. Commercially feasible micropropagation of mountain laurel, (Kalmia latifolia), by use of shoot tip culture. Combined Proceedings: International Plant Propagator´s Society, n. 30, p. 421-427, 1981.
Lopes, S. F. et al. Caracterização ecológica e distribuição diamétrica da vegetação arbórea em um remanescente de floresta estacional semidecidual na Fazenda Experimental do Glória, Uberlândia, MG. Bioscience Journal, v. 27, p. 322-335, 2011.
Lorenzi, H. (Ed). Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Nova Odessa, SP: Editora Plantarum, 2014. 388 p.
Malosso, M. G. et al. Micropropagation and in vitro conservation of Jacaranda decurrens Cham. Journal of Medicinal Plants Research, v. 6, n. 7, p. 1147-1154, 2012. http://dx.doi.org/10.5897/JMPR11.436.
Miranda, N. A. et al. Meio de cultura, reguladores de crescimento e formas de vedação de tubos de ensaio na multiplicação in vitro de candeia (Eremanthus incanus (Less.) Less). Scientia Forestalis, v. 44, n. 112, p. 1009-1018, 2016. http://dx.doi.org/10.18671/scifor.v44n112.22.
Murashige, T. & Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, v. 15, p. 473-497, 1962. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x.
Nagori, R. & Purohi, S. D. In vitro plantet regeneration in Annona squamosa thought direct shoot but differentiation on hypocotyl segmen ts. Scientia Horticulturae, v. 99, p. 89-98, 2004. http://dx.doi.org/10.1016/S0304-4238 (03)00084-0.
Oliveira, L. S. et al. Micropropagação de espécies florestais brasileiras. Pesquisa Florestal Brasileira, v. 33, n. 76, p. 439-453, 2013. http://dx.doi.org/10.4336/2013.pfb.33.76.481.
Pinhal, H. F. et al. Aplicações da cultura de tecidos vegetais em fruteiras do Cerrado. Ciência Rural, v. 41, n. 7, p. 1136-1142, 2011. http://dx.doi.org/10.1590/S0103-84782011005000089.
Rodrigues, M. R. et al. Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.). In vitro Cellular & Developmental Biology: Plant, v. 48, n. 1, p. 67-72, 2012. http://dx.doi.org/10.1007/s11627-011-9398-8.
Socolowski, F. et al. Viability of recently harvested and stored Xylopia aromatica (Lam.) Mart. (Annonaceae) seeds. Revista Brasileira de Sementes, v. 34, n. 3, p. 408-415, 2012. http://dx.doi.org/10.1590/S0101-31222012000300007.
Xavier, A. et al. (Ed.). Silvicultura clonal: princípios e técnicas. Viçosa, MG: Ed. da UFV, 2013. 279 p.
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Published
2019-07-12
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PORFÍRIO, Kennedy de Paiva; TITON, Miranda; CASTRO, Ana Caroline Macedo de; PEREIRA, Israel Marinho; KNEGT, Rafael Antonius Pfeilsticker de. In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations. Pesquisa Florestal Brasileira, [S. l.], v. 39, n. 1, 2019. DOI: 10.4336/2019.pfb.39e201901895. Disponível em: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1895. Acesso em: 17 may. 2024.
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