In vitro antagonism of Trichoderma species against forest phytopathogenic fungi

Authors

DOI:

https://doi.org/10.4336/2023.pfb.43e202002140c

Keywords:

Biological control, Biocontrol, Bioactive properties

Abstract

The objective of this work was to evaluate the potential of Trichoderma isolates to control phytopathogenic fungi of forest importance. The tests were performed with Alternaria sp., Botrytis cinerea, Cylindrocladium candelabrum, Pestalotiopsis sp. and Sclerotium rolfsii. We used Trichoderma spp. collected from soils in Brunópolis, Rio do Sul and Curitibanos in Santa Catarina State, and in São Paulo, SP and from a commercial product (positive control). Pairing culture technique was carried out and evaluations were conducted out through daily measurements. The experimental design was completely randomized, with 5 replications. The area under the disease progress curve and the percentage of inhibition were calculated. The data were submitted to analysis of variance and Tukey´s test at 5% probability. In all treatments, mycelial growth differed from the absolute control, except for the fungus S. rolfsii where only the Curitibanos´ isolate showed inhibition. For the other pathogens, inhibition ranged from 46.8% to 75.5%. Regarding the diameter of the colonies, the inhibition varied from 56.2% to 87.0%. The isolates of the genus Trichoderma have high potential as inhibitors of some phytopathogens species.

Downloads

Download data is not yet available.

Author Biographies

Riani Grimes, Universidade Federal de Santa Catarina, Curso de Engenharia Florestal

http://lattes.cnpq.br/2971716609567570

Gabriela Carolina dos Santos, Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Ecossistemas Agrícolas e Naturais

http://lattes.cnpq.br/5761388334800435

André Luiz Graf Júnior, Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Ecossistemas Agrícolas e Naturais

http://lattes.cnpq.br/2844908922858801

João Batista Tolentino Júnior, Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Ecossistemas Agrícolas e Naturais

http://lattes.cnpq.br/3223191297538885

Adriana Terumi Itako, Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Ecossistemas Agrícolas e Naturais

http://lattes.cnpq.br/5323223701627519

References

Agrofit. Sistema de agrotóxicos fitossanitários. Disponível em: http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons. Acesso em: 20 set. 2021.

Alfenas, A. C. & Mafia, R. G. Métodos em fitopatologia. Viçosa, MG: UFV, 2007. 382 p.

Auer, C. G. & Santos, Á. F. dos. Doenças em eucaliptos destinados à produção de energia na região Sul do Brasil. Pesquisa Florestal Brasileira, v. 31, n. 68, p. 373-379, 2011. https://doi.org/10.4336/2011.pfb.31.68.373.

Auler, A. C. V. et al. Antagonismo de Trichoderma harzianum a Sclerotium rolfsii nas culturas do feijoeiro e soja. Revista Agro@Mbiente on-Line, v. 7, n. 3, p. 359-365, 2013. http://dx.doi.org/10.18227/1982-8470ragro.v7i3.1335.

Báez-Vallejo, N. et al. Forest tree associated bacteria for potential biological control of Fusarium solani and of Fusarium kuroshium, causal agent of Fusarium dieback. Microbiological Research, v. 235, p. 1-13, 2020. http://dx.doi.org/10.1016/j.micres.2020.126440.

Barman, H. et al. Evaluation of plant products and antagonistic microbes against grey blight (Pestalotiopsis theae), a devastating pathogen of tea. African Journal of Microbiology Research, v. 9, n. 18, p. 1263-1267, 2015. https://doi.org/10.5897/AJMR2015.7391.

Carvalho Filho, M. R. et al. Biological Control of leaf spot and growth promotion of eucalyptus plants by Trichoderma spp. Journal of Agricultural Science, v. 10, n. 9, p. 459-467, 2018. https://doi.org/10.5539/jas.v10n9p459.

Cassiolato, A. M. R. et al. Ação de mutantes de Trichoderma harzianum na formação, germinação carpogênica de escleródios de Sclerotinia sclerotiorum e sobrevivência de plantas de alface. Fitopatologia Brasileira, v. 22, n. 1, p. 34-38, 1997.

El-Debaiky, S. A. Antagonistic studies and hyphal interactions of the new antagonist Aspergillus piperis against some phytopathogenic fungi in vitro in comparison with Trichoderma harzianum. Microbial Pathogenesis, v. 113, p. 135-143, 2017. https://doi.org/10.1016/j.micpath.2017.10.041.

Fernandez-Winzer, L. et al. Direct and indirect community effects of the invasive plant pathogen Austropuccinia psidii (myrtle rust) in eastern Australian rainforests. Biological Invasions, v. 22, n. 7, p. 2357-2369, 2020. https://doi.org/10.1007/s10530-020-02260-2.

IBGE. Instituto Brasileiro de Geografia e Estatística. Produção Agrícola. Produção agrícola. Disponível em: https://cidades.ibge.gov.br/brasil/sc/curitibanos/pesquisa/14/0. Acesso em: 20 set 2021.

Isaias, C. O. et al. Ação antagônica e de metabólitos bioativos de Trichoderma spp. contra os patógenos Sclerotium rolfsii e Verticillium dahliae. Summa Phytopathologica, v. 40, n. 1, p. 34-41, 2014. https://doi.org/10.1590/s0100-54052014000100005.

Maciel, C. G. et al. Trichoderma spp no biocontrole de Cylindrocladium candelabrum em mudas de Eucalyptus saligna. Revista Árvore, v. 36, n. 5, p. 825-832, 2012. https://doi.org/10.1590/S0100-67622012000500004.

Mafia, R. G. et al. Tombamento de mudas de espécies florestais causado por Sclerotium rolfsii Sacc. Revista Árvore, v. 31, n. 4, p. 629-634, 2007. https://doi.org/10.1590/s0100-67622007000400007.

Matrood, A. A. A. et al. Synergistic interaction of Glomus mosseae T. and Trichoderma harzianum R. in the induction of systemic resistance of Cucumis sativus L. to Alternaria alternata (Fr.) K. Plant Science Today, v. 7, n. 1, p. 101-108, 2020.

Mazrou, Y. S. A. et al. Antagonistic activity and molecular characterization of biological control agent Trichoderma harzianum from Saudi Arabia. Egyptian Journal of Biological Pest Control, v. 30, n. 4, p. 1-8, 2020. https://doi.org/10.1186/s41938-020-0207-8.

Medrado, P. H. da S. Fungos de solo no controle biológico de fitopatógenos. 2019. 103 f. Dissertação (Mestrado em Ecossistemas Agrícolas e Naturais) - Universidade Federal de Santa Catarina, Curitibanos.

Meyer, M. C. et al. (ed.). Trichoderma: uso na agricultura. Brasília, DF: Embrapa, 2019. 538 p.

Nascimento, J. M. et al. Inibição do crescimento micelial de Cercospora calendulae Sacc. por extratos de plantas medicinais. Revista Brasileira de Plantas Medicinais, v. 15, n. 4, p. 751-756, 2013.

Oliveira, G. M. et al. Levantamento de fungos em plantas nativas da Caatinga. Revista Brasileira de Geografia Física, v. 7, n. 3, p. 458-465, 2014.

Sáa, M. N. F. de et al. Efeito de Bacillus sp. e Trichoderma sp. no crescimento micelial de Sclerotium rolfsi. Acta Brasiliensis, v. 3, n. 2, p. 79-81, 2019.

Saju, K. A. et al. In vitro evaluation of biocontrol agents, botanicals and fungicides against Pestalotiopsis sp . infecting large cardamom (Amomum subulatum Roxb .). Journal of Spices and Aromatic Crops, v. 20, n. 2, p. 89-92, 2011.

Santopuoli, G. et al. Supporting policy decision makers in the establishment of forest plantations, using SWOT analysis and AHPs analysis: a case study in Tocantins (Brasil). Land Use Policy, v. 54, p. 549-558, 2016.

Santos, Ã. F. dos & Bora, K. C. Detecção de Sclerotium rolfsii em sementes e sua patogenicidade em mudas de palmeira real. Colombo: Embrapa Florestas, 2011. 2 p.

Santos, G. S. et al. Stem rot of eucalyptus cuttings caused by Neopestalotiopsis spp. in Brazil. Journal of Phytopathology, v. 168, n. 6, p. 311-321, 2020. https://doi.org/10.1111/jph.12894.

Sbravatti Júnior, J. A. et al. Seleção in vitro de fungos endofíticos para o controle biológico de Botrytis cinerea em Eucalyptus benthamii. Floresta, v. 43, n. 1, p. 145-152, 2013.

Schultz, B. et al. Impacto da mancha foliar causada por Cylindrocladium candelabrum em plantios jovens de Eucalyptus benthamii em Rio Negrinho - SC. Ciência Florestal, v. 25, n. 2, p. 307-316, 2015.

Shaner, G. & Finney, R. E. The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology, v. 67, p. 1051-1056, 1977.

Singh, V. et al. Isolation and screening of high salinity tolerant Trichoderma spp. with plant growth property and antagonistic activity against various soilborne phytopathogens. Archives of Phytopathology and Plant Protection, v. 52, n. 7-8, p. 667-680, 2019. https://doi.org/10.1080/03235408.2019.1648917.

Sridharan, A. P. et al. Comprehensive profiling of the VOCs of Trichoderma longibrachiatum EF5 while interacting with Sclerotium rolfsii and Macrophomina phaseolina. Microbiological Research, v. 236, p. 1-13, 2020. https://doi.org/10.1016/j.micres.2020.126436.

Zauza, E. Á‚. V. & Ferreira, M. A. Manejo integrado de doenças bióticas em viveiros florestais. In: Santos, B. A. et al. (ed.). Patologia florestal: desafios e perspectivas. São Carlos, SP: Suprema, 2013. p. 103-117.

Published

2023-07-07

How to Cite

GRIMES, Riani; SANTOS, Gabriela Carolina dos; GRAF JÚNIOR, André Luiz; TOLENTINO JÚNIOR, João Batista; ITAKO, Adriana Terumi. In vitro antagonism of Trichoderma species against forest phytopathogenic fungi. Pesquisa Florestal Brasileira, [S. l.], v. 43, 2023. DOI: 10.4336/2023.pfb.43e202002140c. Disponível em: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/2140. Acesso em: 17 may. 2024.

Issue

Section

Articles

Most read articles by the same author(s)

Similar Articles

<< < 3 4 5 6 7 8 9 10 11 12 > >> 

You may also start an advanced similarity search for this article.