Effect of different culture periods for Luehea divaricata on micropropagation from shoots
DOI:
https://doi.org/10.4336/2021.pfb.41e201901921Keywords:
Açoita-cavalo, In vitro culture, SenescenceAbstract
The objective of the present study was to evaluate the effect of different in vitro culture periods of micropropagated Luehea divaricata shoots. The treatments were constituted by different in vitro culture periods (14; 21; 28; 40; 56; 70; 84; 102; 116; 130; 154, 186 or 200 days), without subculture or transfer to a new nutritive medium. The survival and establishment reached around 90% up to 84 days. After that, the mortality increased. Culture period of 56 days was the one that showed the most appropriate proportion of number of leaves and senescent leaves, simultaneously, showing nine leaves and only one senescent. Averages above 50% were observed for primary roots formation after 70 days of in vitro culture, providing the best results (60%) at 200 days. For secondary roots, the highest averages were observed after 102 days (41.5%), reaching 45.3% at 200 days. The micropropagation of Luehea divaricata is significantly affected by the in vitro culture period, which can be extended up to approximately 60 days. From that period, high mortality and leaf senescence are registered.
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Araujo, M. M. et al. Produção de sementes e mudas: um enfoque à silvicultura. Santa Maria, RS: Ed. UFSM, 2018. 448 p.
Barria, M. J. Etileno em plantas superiores: síntese e propriedades fisiológicas. In: Barrueto Cid, L. P. Hormônios vegetais em plantas superiores. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. v. 01. p. 102-125.
Barrueto Cid, L. P. & Teixeira, J. B. Oxidação fenólica,vitrificação e variação somaclonal. In: Barrueto Cid, L. P. Cultivo in vitro de plantas. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. v. 1, p. 51-66.
Dutra, A. F. et al. Germinação de sementes e emergência de plântulas de Luehea divaricata Mart. et. Zucc. em diferentes substratos. Ciência Florestal, v. 26, n. 2, p. 411-418, 2016. https://doi.org/10.5902/1980509822744.
Esposito-Polesi, N. P. et al. Endophytic bacterial communities associated with two explant sources of Eucalyptus benthamii Maiden & Cambage. World Journal of Microbiology and Biotechnology, v. 31, p. 1737-1746, 2015. http://dx.doi.org/10.1007/s11274-015-1924-0.
Esposito-Polesi, N. P. Contaminação versus manifestação endofítica: implicações no cultivo in vitro de plantas. Rodriguésia, v. 71, p. 1- 15, 2020. http://dx.doi.org/10.1590/2175-7860202071072.
Faganello, L. R. et al. Efeito dos ácidos indolbutírico e naftalenoacético no enraizamento de estacas semilenhosas de Cordia trichotoma (Vell.) Arrab. ex Steud. Ciência Florestal, v. 25, n. 4, p. 863-871, 2015. https://doi.org/10.5902/1980509820584.
Fermino Junior, P. C. P. & Scherwinski-Pereira J. E. Germinação e propagação in vitro de cerejeira (Amburana acreana (Ducke) A.C. Smith - Fabaceae). Ciência Florestal, v. 22, n 1, p. 1-9, 2012. https://doi.org/10.5902/198050985074.
Ferreira, D. F. Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, v. 38, n. 2, p 109-112, 2014. https://doi.org/10.1590/S1413-70542014000200001.
Flôres, A.V. et al. Estabelecimento e multiplicação in vitro de Luehea divaricata Mart. & Zucc. Ciência Florestal, v. 21, n. 1, p. 175-182, 2011. https://doi.org/10.5902/198050982760.
Murashige, T. & Skoog, F. K. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v. 15, p. 473-497, 1962.
Oliveira, L. M. et al. Efeito de citocininas na senescência e abscisão foliar durante o cultivo in vitro de Annona glabra L. Revista Brasileira de Fruticultura, v. 29, n. 1, p. 25-30, 2007. https://doi.org/10.1590/S0100-29452007000100008.
Oliveira, L. S. et al. Micropropagação de espécies florestais brasileiras. Pesquisa Florestal Brasileira, v. 33, n. 76, p. 445-460, 2013. https://doi.org/10.4336/2013.pfb.33.76.481.
Pasa, M. S. et al. Qualidade de luz e fitorreguladores na multiplicação e enraizamento in vitro da amoreira-preta "˜Xavante´. Ciência Rural, v. 42, n. 8, p. 1392-1396, 2012. https://doi.org/10.1590/S0103-84782012000800010.
Pimentel-Gomes, F. Curso de estatística experimental. 15. ed. Piracicaba: Livraria Nobel, 2009. 451 p.
Sá, A. J. et al. Sealing and explant types on the mangaba micropropagation. Ciência Agrotécnica, v. 36, n. 4, p. 406-414, 2012. https://doi.org/10.1590/S1413-70542012000400004.
Sartor, F. R. et al. Diferentes meios de cultura e antioxidantes no estabelecimento in vitro do jacarandá da Bahia. Bioscience Journal, v. 29, n. 2, p. 408-411, 2013.
Silva, K. B et al. Rizogênese in vitro em brotações de Luehea divaricata Mart. & Zucc. Ciência Florestal, v. 29, n. 3, p. 1282-1295, 2019. https://doi.org/10.5902/1980509826045.
Silva, K. B. et al. Sucrose and substrates on the acclimatization of micropropagated Luehea divaricata plants. Floresta e Ambiente, v. 27, n. 1, p. 1-9, 2020. https://doi.org/10.1590/2179-8087.117017.
Taiz, L. & Zeiger, E. Fisiologia vegetal. Porto Alegre: Artmed, 2004. 719 p.
Torres, G. R. C. et al. Control of contaminants during introduction and establishment of Bambusa vulgaris in vitro. Research in Biotechnology, v 7, p 58-67, 2016. https://doi.org/10.19071/rib.2016.v7.3056.
Xavier, A. et al. Silvicultura clonal: princípios e técnicas. Viçosa, MG: Universidade Federal de Viçosa, 2013. 279 p.
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Copyright (c) 2021 Karol Buuron da Silva, Lia Rejane Silveira Reiniger, Silvia Machado dos Santos Rabaiolli, Ana Cristina da Fonseca Ziegler, Charlene Moro Stefanel
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