Disinfection and in vitro establishment of Acacia mearnsii nodal segments

Authors

  • Ecléia Alexandra Poltronieri Buda Salles Universidade Federal do Paraná
  • Giovana Bomfim Alcantara Universidade Federal do Paraná http://orcid.org/0000-0001-5578-5843
  • Marguerite Germaine Ghislaine Quoirin Universidade Federal do Paraná
  • Antonio Natal Gonçalves Universidade de São Paulo - Esalq
  • Antonio Rioyei Higa Universidade Federal do Paraná

DOI:

https://doi.org/10.4336/2017.pfb.37.92.1392

Keywords:

Black wattle, Clones, Cutting

Abstract

Due to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival.

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References

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Published

2017-12-29

How to Cite

SALLES, Ecléia Alexandra Poltronieri Buda; ALCANTARA, Giovana Bomfim; QUOIRIN, Marguerite Germaine Ghislaine; GONÇALVES, Antonio Natal; HIGA, Antonio Rioyei. Disinfection and in vitro establishment of Acacia mearnsii nodal segments. Pesquisa Florestal Brasileira, [S. l.], v. 37, n. 92, p. 485–491, 2017. DOI: 10.4336/2017.pfb.37.92.1392. Disponível em: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1392. Acesso em: 17 may. 2024.

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