Metodologia de extração de DNA para análise genética de populações de pupunha (Bactris gasipaes Kunth.)

Ahmad Abdul Latif Hamze; Antonio Nascim Kalil Filho; Valderês Aparecida de Sousa; Ãlvaro Figueredo dos Santos; Edinelson José Maciel Neves

DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations

Authors

Ahmad Abdul Latif Hamze Unesp
Antonio Nascim Kalil Filho Embrapa Florestas
Valderês Aparecida de Sousa Embrapa Florestas
Ãlvaro Figueredo dos Santos Embrapa Florestas
Edinelson José Maciel Neves Embrapa Florestas

Keywords:

Heart-palm, Arecaceae, Protocol

Abstract

DNA extraction is a very important step for DNA genetic analysis in peach palm (Bactris gasipaes Kunth.). The aim of this work was to develop a methodology of DNA extraction. Fresh, frozen and dried leaves were utilized. DNA from fresh leaves were purified one time while frozen and dried leaves was purified two times. DNA bands were coloured intensively showing a high amount of purified DNA and with an excellent resolution in agarosis gel 2%, such for fresh leaves as much as for frozen and dried leaves.

Downloads

Download data is not yet available.

References

GAIOTTO, F. A. Influência sobre herança quantitativa e estrutura genética em populações naturais de Euterpe edulis MART. utilizando marcadores microsatelites. Piracicaba: ESALQ, 2001. 122 p.
SOUSA, N. R. de; RODRIGUES, D. P.; CLEMENT, C. R.; NAGAO, E. O.; ASTOLFI-FILHO, S. Discriminação de raças primitivas de pupunha (Bactris gasipaes) na Amazônia brasileira por meio de marcadores moleculares (RAPDs). Acta Amazônica, v. 3, n. 4, p. 539-545, 2001.
SAWAZAKI, H. E.; BOVI, M. L. A.; SODEK, L.; COLOMBO, C. A. Diversidade genética em palmeiras através de isoenzimas e RAPD. Revista Brasileira de Biologia, v. 58, n. 4, p. 681-691, 1998.

Downloads

PDF (Português (Brasil))

Published

2003-12-30

How to Cite

HAMZE, Ahmad Abdul Latif; KALIL FILHO, Antonio Nascim; SOUSA, Valderês Aparecida de; SANTOS, Ãlvaro Figueredo dos; NEVES, Edinelson José Maciel. DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations. Pesquisa Florestal Brasileira, [S. l.], n. 47, p. 117–122, 2003. Disponível em: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1612. Acesso em: 17 may. 2024.

Download Citation

Issue

No. 47 (2003): jul./dez.

Section

Scientific Notes

License

The BRAZILIAN JOURNAL OF FORESTRY RESEARCH reserves the right to correct grammar, improve clarity, and impose PFB standard although respecting the author's style.

Final proof version will be sent to the correspondence author.

Published papers will become property of the BRAZILIAN JOURNAL OF FORESTRY RESEARCH.

Published papers may be used by the authors, without previous PFB authorization, allowed when source is cited.

Any mention of trademarks or methods do not imply in its recommended by the Editorial Committee.

The authors are the exclusive responsible for opinions and concepts developed in the manuscript.

Most read articles by the same author(s)

Antonio Nascim Kalil Filho, Alexandre Jacobus Lopes, Gizelda Maia Rêgo, Adilson Tomachitz, Evaluation of Physiologic Quality of Imbuya Seeds by Tetrazolium Test , Pesquisa Florestal Brasileira: No. 57 (2008): jul./dez.
Edinelson José Maciel Neves, Soil and climate on the establishment of neem (Azadirachta indica A. Juss) cultivation in forestry and agroforestry systems on different Brazilian regions , Pesquisa Florestal Brasileira: No. 49 (2004): jul./dez.
Rudimar Mafacioli, Alvaro Figueredo dos Santos, Dauri José Tessmann, João Batista Vida, Etiology and Management of the Diseases of Peach Palm in Brazil , Pesquisa Florestal Brasileira: No. 58 (2009): jan./jun.
Michele Strapasson, Ãlvaro Figueredo dos Santos, Antonio Carlos de Souza Medeiros, Seed-Borne Fungi Associated with Schinus terebinthifolius , Pesquisa Florestal Brasileira: No. 45 (2002): jul./dez.
Emerson Gonçalves Martins, Edinelson José Maciel Neves, José Alfredo Sturion, Nicole Duleba, Genetic Gain for Wood Volume in Progeny Trial of Grevillea robusta Cunn (Breeding Cycle 2) in Londrina, Paraná State , Pesquisa Florestal Brasileira: No. 51 (2005): jul./dez.
Edinelson José Maciel Neves, Ãlvaro Figueredo dos Santos, Production of three cuttings of Bactris gasipaes for heart-of-palm under different plant density in the Paraná State Coastal Region, Brazil , Pesquisa Florestal Brasileira: No. 56 (2008): jan./jun.
Emerson Gonçalves Martins, Edinelson José Maciel Neves, José Alfredo Sturion, Hideyo Aoki, Genetic Gain for Wood Volume in Progeny Trial of Grevílea (Breeding Cycle 2) in Avaré, State of São Paulo , Pesquisa Florestal Brasileira: No. 52 (2006): jan./jun.
Antonio Nascim Kalil Filho, Elcio Hirano, José Alfredo Sturion, Valderês Aaparecida de Sousa, Luiz Gustvo Marzollo, Filipe Nicolotti, Daniele Ukan, (Ocotea porosa Ness et Martius ex. Nees, Lauraceae) from populations of mixed of mixed Ombrophylous forest , Pesquisa Florestal Brasileira: No. 49 (2004): jul./dez.
Edinelson José Maciel Neves, Ãlvaro Figueredo dos Santos, Emerson Gonçalves Martins, Sérgio Ahrens, Antonio Nascim Kalil Filho, Effects of different spacings on heartof- palm production from peach-palm planted in the coastal region of the state Paraná - Brazil - 1ST Harvest , Pesquisa Florestal Brasileira: No. 46 (2003): jan./jun.
Antonio Nascim Kalil Filho, Valderês Aparecida de Sousa, Luiz Gustavo Marzollo, Elcio Hirano, Dynamics of seeds germination of imbuya (Ocotea porosa Nees et Martius ex. Nees, Lauraceae ) progenies within populations of Paraná and Santa Catarina , Pesquisa Florestal Brasileira: No. 48 (2004): jan./jun.

<< < 1 2 3 4 > >>

DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations

Authors

Keywords:

Abstract

Downloads

References

Downloads

Published

How to Cite

Issue

Section

License

Most read articles by the same author(s)

Similar Articles

Language

Make a Submission

Keywords

indexacao

Redes Sociais

Announcements

PFB at Social Networking