Minimum growth in vitro and resumed growth of Luehea divaricata shoots
DOI:
https://doi.org/10.4336/2021.pfb.41e201902026Keywords:
Plant genetic resources, Germplasm, Tissue cultureAbstract
The objective of this work was to evaluate the effect of sorbitol and on the growth period on minimum growth and subsequent resumed growth of Luehea divaricata shoots. Two tests were performed, one of minimal growth and the other of resumed growth. In the presence of sorbitol at minimum growth there was a reduction in averages of survival, establishment, number of leaves and primary and secondary roots. When the period was considered, it was observed a reduction at 30 days followed by increase and stabilization in averages of primary and secondary roots, and number of leaves and of shoots. The presence of sorbitol promoted a significant reduction in averages, in the resumed growth, for survival, establishment, number of leaves and of shoots. A decrease of averages in survival and establishment was observed only after 120 days. The presence of sorbitol is important for in vitro conservation of L. divaricata by minimum growth, which can be carried out for up to 120 days. The subsequent resumed growth of the shoots preserved in the presence of sorbitol is limited to 90 days.
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