DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations
Keywords:
Heart-palm, Arecaceae, ProtocolAbstract
DNA extraction is a very important step for DNA genetic analysis in peach palm (Bactris gasipaes Kunth.). The aim of this work was to develop a methodology of DNA extraction. Fresh, frozen and dried leaves were utilized. DNA from fresh leaves were purified one time while frozen and dried leaves was purified two times. DNA bands were coloured intensively showing a high amount of purified DNA and with an excellent resolution in agarosis gel 2%, such for fresh leaves as much as for frozen and dried leaves.
Downloads
References
SOUSA, N. R. de; RODRIGUES, D. P.; CLEMENT, C. R.; NAGAO, E. O.; ASTOLFI-FILHO, S. Discriminação de raças primitivas de pupunha (Bactris gasipaes) na Amazônia brasileira por meio de marcadores moleculares (RAPDs). Acta Amazônica, v. 3, n. 4, p. 539-545, 2001.
SAWAZAKI, H. E.; BOVI, M. L. A.; SODEK, L.; COLOMBO, C. A. Diversidade genética em palmeiras através de isoenzimas e RAPD. Revista Brasileira de Biologia, v. 58, n. 4, p. 681-691, 1998.
Downloads
Published
How to Cite
Issue
Section
License
The BRAZILIAN JOURNAL OF FORESTRY RESEARCH reserves the right to correct grammar, improve clarity, and impose PFB standard although respecting the author's style.
Final proof version will be sent to the correspondence author.
Published papers will become property of the BRAZILIAN JOURNAL OF FORESTRY RESEARCH.
Published papers may be used by the authors, without previous PFB authorization, allowed when source is cited.
Any mention of trademarks or methods do not imply in its recommended by the Editorial Committee.
The authors are the exclusive responsible for opinions and concepts developed in the manuscript.