Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds

Abstract

The aim of this experiment was to evaluate the best concentration  and the incubation time to test germination in seeds of  Anadenanthera peregrina (L.) Speg. The seeds were  preconditioned at 25 ºC for 14 hours immersed in distilled water.  The coat were removed and the embryos submerged in tetrazolium solution (pH 6.5) solution in the concentrations of  0.1%; 0.05% and 0.01%, being kept in dark at 25 ºC for three and five hours and at 30 ºC for 24 hours. It was evaluate the intensity and localization of the coloration, presence of milky areas, tissues aspect. The embryos were classified in nine classes of viability. The best treatment as substitute of the germination test was the 0.1% tetrazolium incubated at 30 ºC for 24 hours. Viable seeds of A. peregrine were classified in classes 1, 2 and 3.

doi: 10.4336/2011.pfb.31.67.269